 หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : In vitro Preservation of Sugarcane Callus by Using Daminozide) ผู้เขียน: Rongrong Visessuwan,  ดร.เรวัต เลิศฤทัยโยธิน, รองศาสตราจารย์ สื่อสิ่งพิมพ์:pdf AbstractCallus was induced from 2 cultivars and 1 line of sugarcane by culture shoot tip on Murashige and Skoog medium supplemented with 3 mg/l 2, 4-dichlorophenoxy acetic acid and 15% by volume of coconut water. This callus was cultured on MS medium supplemented with 1 mg/l 2, 4-D, 15% by volume of coconut water and various concentration of daminozide for 5 months. Callus cultured on medium with 500 mg/l daminozide could develop to be plantlets, while callus cultured on medium with 1000, 1500 and 2000 mg/l daminozide could not develop to be plantlets. By using daminozide, callus of sugarcane could be stored for 5 months by subculture only 1 time after culture for 2 months. |
หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : In Vitro Preservation of Sugarcane Plantlets under Low Temperature ) ผู้เขียน:ดร.เรวัต เลิศฤทัยโยธิน, รองศาสตราจารย์, Pongpeng Chit-areerat, Rongrong Visessuwan สื่อสิ่งพิมพ์:pdf AbstractPlantlets of two cultivated sugarcane cultivars, Supan 1 and C02, cultured on modified Murashige and Skoog supplemented with 1 mg/I 2,4-dichlorophenoxy acetic acid, 0.1 mg/1 myo-inositol, 0.02mg/1 glycine, 20g/l sucrose and 10% by volume of coconut water (CD media) were used. Plantlets were transferred to incubator controlled at 5?C and 15?C under 24 hours light condition for I and 2 months. After the storage periods, plantlets were cultured under room temperature for I month. Culture under 5?C for 1 month and 15?C for 1 and 2 months could decrease growth of sugarcane plantlets in which all of plantlets could normally rcgrowth under room temperature. Plantlets of sugarcane cultivar Supan I cultured under 5?C for 2 months could. regrowth only 50-60% under room temperature. |
หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Regeneration of Seeda Tomato Plants from Isolated Protoplasts) ผู้เขียน:Praparat Kalayawudhipong, ดร.สุพัฒน์ อรรถธรรม, ศาสตราจารย์ , Rongrong Visessuwan สื่อสิ่งพิมพ์:pdf AbstractGreenhouse grown and tissue cultured Seeda tomato plants were used as leaf tissue source for protoplast isolation. Yields of 4.3 x 106 and 8.3 x 104 protoplasts/g fresh tissue were obtained from greenhouse grown and tissue cultured plants respectively. Only protoplasts from field grown plants divided, developed microcalli and regenerated the whole plant. |
หัวเรื่อง:การเพาะเลี้ยงเนื้อเยื่อปลายยอดอ้อยให้พัฒนาเป็นต้นพืชที่สมบูรณ์ ผู้เขียน:Kriuk Naritoom, นายเกษม สุขสถาน, ผู้ช่วยศาสตราจารย์ , Vichai Korpradittskul, Isara Suksathan, Rongrong Visessuwan, ดร.นงณพชร คุณากร , นางสุภาพร กลิ่นคง, รองศาสตราจารย์ , Monthar Suriyachaiyakorn สื่อสิ่งพิมพ์:pdf AbstractSugarcane shoot tips were cultured in liquid media continuously stirred in circular manner by a gyratory shaker rotated at 2 cycles/min under continuous illumination of 2000-4000 lux. It was found that the MS me?dium supplemented with 0.2 mg/1 BA and 0.02 mg/1 NAA gave the best proliferation rate, 4-5 shootlets could be obtained within 20 days from development of axillary buds. After 50 days the shootlets were multiplied at the rate of 4 folds in every 2 weeks by subculturing into the same medium The resulting shootlets could be progres?sively increased at the rate of 6 folds in every 4 weeks when transferred on to an MS solid medium, supple?mented with 0.2 mg/l BA and 0.02 mg/1 NAA before transferred on to MS medium containing 15oh (v/v) coco?nut water and 1 mg/ l 2,4-D to enhance the shoots. Rooting of the shoots were successfully done on a MS me?dium with 1 mg/I IBA. |
หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Production of Vieus-Free Sugarcane by Tissue Culture) ผู้เขียน:Rongrong Visessuwan, Wichai Korpraditskul, ดร.สุพัฒน์ อรรถธรรม, ศาสตราจารย์, นางสุภาพร กลิ่นคง, รองศาสตราจารย์ สื่อสิ่งพิมพ์:pdf AbstractSugarcane plants of the three commercially varieties namely F140, Q83 and Supan I were produced free from sugarcane mosaic virus by the apical meristem culture. Shoot tips, 0.2-0.5 mm. in size, were cultured on MS medium supplemented with 3 mg/L 2,4-4 and 15% (v/v) coconut water. Yellowish-white callus developed within 2 months. Plantlets were regenerated from calli cutured on MS medium with 1 mg/L 2,4-D and 15% (v/v) coconut water. The suitable medium for root induction in all cultivars was the MS or MS with 1 mg/L IBA. Detection of sugarcane mosaic virus (SCMV) in plantlets or individual plants by ELISA showed negative result suggesting that they were virus free. |
หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : In vitro Preservation of Sugarcane Callus under Low Temperature) ผู้เขียน:ดร.เรวัต เลิศฤทัยโยธิน, รองศาสตราจารย์, Rongrong Visessuwan, Kriuk Naritoom สื่อสิ่งพิมพ์:pdf AbstractCallus was induced from commercial cultivar of sugarcane by culture shoot tip on Murashige and Skoog medium supplemented with 3 mg/1/2, 4-dichlorophenoxyacetic acid and 15% by volume of coconut water. This callus was preserved under low temperature at 1 and 5 degree C with different periods of preservation, and then was cultured at 27 degree C for 6 months. After preservation under low temperature, plantlets could be induced from callus preserved at 5 degree C but could not be induced from callus preserved at 0 degree C. In addition, at 5 degree C callus could develop to be plantlets only when preserved not longer than 30 days. Callus preserved at low temperature grew slower than callus not preserved at low temperature. |